Complement receptors: Specific detection by molecular complexes

Tissue-specific phosphorylation of complement receptors CR1 and CR2

CR1 of neutrophils and monocytes may exist in a resting state, in which it only binds ligand-coated particles, or an activated state, in which it mediates phagocytosis. Because the activated state of CR1 can be induced by the stimulation of protein kinase C with PMA, CR1 was assessed for phosphorylation. Purified human neutrophils, monocytes, eosinophils, tonsilar lymphocytes, SB cells, and ery...

Detection of rotavirus by Latex Agglutination Test (Rotales); Comparison with Electron Microscopy and Complement Fixation Test

Monoclonal Antibodies against Complement 3 Neoantigens for Detection of Immune Complexes and Complement Activation

C3-bearing immune complexes and C3 activation products were detected by using two monoclonal antibodies, one specific for a neoantigenic determinant on C3c and the other for C3d. To quantitate immune complexes, the anti-C3c or anti-C3d antibodies were fixed to microtiter plates and reacted with test plasma. The binding of C3-bearing immune complexes in this plasma was then measured with radiois...

The Detection of Massive Molecular Complexes

We have imaged the kpc scale distribution of CO(J=1-0) emission in the ring galaxy system Arp 143 (NGC 2444/2445) using the OVRO millimeter array. We find two giant molecular complexes in the ring component (NGC 2445) and a bright central source. The ring complexes represent 20−60% of the detected MH2 , depending on the relative ICO–NH2 for the ring and nucleus. Their individual H2 masses and s...

Complement Receptors in Inflammation

Complement was first discovered in 1889 as a bactericidal protein, distinct from heat stable antibodies present in normal serum. Since that time, it has been shown that the complement system is a biochemical cascade, comprised of more than 30 fluid phase and membraneassociated proteins, normally present as inactive forms. The complement system can be activated by different sequential cascades o...